Education, tips and tricks to help you conduct better fMRI experiments.
Sure, you can try to fix it during data processing, but you're usually better off fixing the acquisition!

Saturday, March 24, 2018

FMRI data modulators 3: Low frequency oscillations - part I


Low frequency oscillations (LFOs) may be one of the the most important sources of signal variance for resting-state fMRI. Consider this quote from a recent paper by Tong & Frederick:
"we found that the effects of pLFOs [physiological LFOs] dominated many prominent ICA components, which suggests that, contrary to the popular belief that aliased cardiac and respiration signals are the main physiological noise source in BOLD fMRI, pLFOs may be the most influential physiological signals. Understanding and measuring these pLFOs are important for denoising and accurately modeling BOLD signals."

If true, it's strange that LFOs aren't higher on many lists of problems in fMRI. They seem to be an afterthought, if thought about at all. I suspect that nomenclature may be partly responsible for much of the oversight. A lot of different processes end up in the bucket labeled "LFO." The term is used differently in different contexts, with the context most often defined by the methodology under consideration. Folks using laser Doppler flow cytometry may be referring to something quite different than fMRI folks. Or not. Which rather makes my point. In this post I shall try to sort the contents of the LFO bucket, and in at least one later post, I shall dig more deeply into "systemic LFOs." These are the LFOs having truly physiological origin; where the adjective is used according to its physiological definition:


The description I pulled up from the Google dictionary tells us the essential nature of systemic LFOs: at least some of them are likely to involve the blood gases. And I'll give you a clue to keep you interested. It's the CO component that may end up being most relevant to us.


What exactly do we mean by low frequency oscillations anyway?

"Low frequency" generally refers to fluctuations in fMRI signal that arise, apparently spontaneously, with a frequency of around 0.1 Hz. The precise range of frequencies isn't of critical importance for this post, but it's common to find a bandwidth of 0.05 - 0.15 Hz under discussion in the LFO literature. I'll just say ~ 0.1 Hz and move on. I added "apparently spontaneously" as a caveat because some of mechanisms aren't all that spontaneous, it turns out.

For the purposes of this post we're talking about variations in BOLD signal intensity in a time series with a variation of ~ 0.1 Hz. There may be other brain processes that oscillate at low frequencies, such as electrical activity, but here I am specifically concerned with processes that can leave an imprint on a BOLD-contrasted time series. Thus, neurovascular coupling resulting in LFO is relevant, whereas low frequency brain electrical activity per se is not, because the associated magnetic fields (in the nanotesla range, implied from MEG) are far too small to matter.

Is LFO the lowest modulation of interest? No. There are physiological perturbations that arise at even lower frequencies. These are often termed very low frequency oscillations (VLFOs) because, well, we scientists are an imaginative bunch. These VLFOs generally happen below about 0.05 Hz. The biological processes that fluctuate once or twice a minute may well be related to the LFOs that are the focus here, but I am going to leave them for another day.

Friday, March 2, 2018

Monitoring gradient cable temperature


While the gradient set is water-cooled, the gradient cables and gradient filters still rely upon air cooling in many scanner suites, such as mine. In the case of the gradient filters, the filter box on my Siemens Trio came with an opaque cover, which we replaced with clear plastic to allow easy inspection and temperature monitoring with an infrared (IR) thermometer:

The gradient filter box in the wall behind my Siemens Trio magnet. It's up at ceiling height, in the lowest possible stray magnetic field. The clear plastic cover is custom. The standard box is opaque white.


Siemens now has a smoke detector inside the gradient filter box, after at least one instance of the gradient filters disintegrating with excess heat. Still, a clear inspection panel is a handy thing to have.

The gradient cables between the filter box and the back of the magnet can also decay with use. If this happens, the load experienced by the gradient amplifier changes and this can affect gradient control fidelity. (More on this below.) The cables can be damaged by excess heat, and this damage leads to higher resistance which itself produces more heating. A classic feedback loop!


The Fluke 561 IR thermometer and a K type thermocouple, purchased separately.